Gene expression profiles of RGS-overexpressing HSPCs. Expression microarray was performed on control or RGS1-, RGS13-, and RGS16-overexpressing CD34⁺ cells (n = 4). (A) Venn diagram showing the number of significantly deregulated genes in RGS-overexpressing cells. (B) Heat map showing the 36 differentially expressed genes commonly altered by the 3 RGS members. (C) Top 10 enriched gene ontology (biological processes) of RGS-regulated genes. (D) Differential expression of 14 target genes with relevance to stem cell trafficking/maintenance was validated by RT-qPCR (n = 8). Statistics: 2-tailed, paired Student t test. **P < .01; ***P < .001.