Transduction of primary human monocytes with BRAFV600E induces epigenetic changes in TI and enhances cytokine production. ChIP polymerase chain reaction showing the percentage of H3K4me3 at promoters and the percentage of H3K27Ac at distal enhancers of genes encoding cytokines: H3K4me3 on IL6 (A); H3K27Ac on IL6 (C); H3K4me3 on TNFA (B); and H3K27Ac on TNFA (D). Monocytes were cultured in 3 different conditions: nontransduced (UT, isolated primary monocytes), transduced with wtBRAF (WT), and transduced with BRAFV600E (VE). Data are the mean ± SEM (n = 6). *P < .05, 1-way analysis of variance. (E-F) Cytokine production by macrophages at baseline and after stimulation with LPS: IL-6 (E); TNFα (F). Data are the mean ± SEM (n = 4); biological duplicates were performed for each condition. *P < .05; **P < .01, Student t test. ChIP, chromatin immunoprecipitation.