Interleukin levels in case of clinical suspicion of PVRL. (A) IL-10 and IL-6 levels in the aqueous humor and vitreous for PVRL screening and diagnosis. In the aqueous humor, an absolute level of IL-10 ≥50 pg/mL and IL-10/IL-6 ratio >1 is suggestive of PVRL (IL-10 cutoff: sensitivity, 89%; specificity, 93%). In patients with uveitis, the level of IL-10 is low and IL-10/IL-6 ratio is <1. In patients with an infectious disease, the IL-10 level might exceed 50 pg/mL, but the IL-6 level might also be >50 pg/mL; the IL-10/IL-6 ratio is <1. In the vitreous, the absolute level of IL-10 ≥400 pg/mL and IL-10/IL-6 ratio >1 are also suggestive of PVRL (IL-10 cutoff: sensitivity, 80%; specificity, 99%). Data adapted.^12,100 (B) The Interleukin Score for Intraocular Lymphoma Diagnosis (ISOLD) is a probability score for the diagnosis of PVRL based on a mathematical formula combining both IL-10 and IL-6 levels in vitreous or aqueous samples with high sensitivity and specificity (93% and 95%, respectively). Data adapted.³⁷