HDAC5 knockdown leads to increased apoptosis caused by activation of the p53 pathway. (A) Growth curves of cells transduced with lentivirus containing luciferase-shRNA or HDAC5-shRNAs. (B) Representative flow cytometry profiles of apoptosis as assessed by dual staining of annexin V and 7AAD at day 13 of culture. (C) Quantitative analysis of apoptosis from 3 independent experiments. (D) Representative western blot analyses of molecules, as indicated on day 11 cultured erythroblasts. (E) Quantitative analysis of the western blot results from 3 independent experiments. (F) qRT-PCR analyses of the molecules as indicated on day 11 cultured erythroblasts. (A,C,E-F) *P < .05; **P < .01; ***P < .001; NS, nonsignificant. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.