Figure 3.
Rpl5+/− newborn mice have macrocytic anemia. Newborn mice (days 1-3) were euthanized and the blood counts analyzed (WT, n = 10; Rpl5+/−, n = 7). Rpl5+/− mice exhibited evidence of anemia (A) with significantly lower hemoglobin (Hb) levels and (B) macrocytosis, as demonstrated by an elevated mean corpuscular volume (MCV). There was no significant (NS) difference in WBC (C) or platelet (PLT) counts (D) between Rpl5+/− and WT mice. The horizontal line represents the median value for each group. **P < .01; ****P < .0001. Newborn livers (days 1 and 2) were isolated, and flow cytometry was performed to analyze erythroid differentiation. There was no difference in liver cellularity (E) but all Rpl5+/− mice had paler livers (F) with decreased Ter119 mean fluorescence intensity (G). (H-I) There was no difference in the number of proerythroblasts (WT, n = 3; Rpl5+/−, n = 3).

Rpl5+/− newborn mice have macrocytic anemia. Newborn mice (days 1-3) were euthanized and the blood counts analyzed (WT, n = 10; Rpl5+/−, n = 7). Rpl5+/− mice exhibited evidence of anemia (A) with significantly lower hemoglobin (Hb) levels and (B) macrocytosis, as demonstrated by an elevated mean corpuscular volume (MCV). There was no significant (NS) difference in WBC (C) or platelet (PLT) counts (D) between Rpl5+/− and WT mice. The horizontal line represents the median value for each group. **P < .01; ****P < .0001. Newborn livers (days 1 and 2) were isolated, and flow cytometry was performed to analyze erythroid differentiation. There was no difference in liver cellularity (E) but all Rpl5+/− mice had paler livers (F) with decreased Ter119 mean fluorescence intensity (G). (H-I) There was no difference in the number of proerythroblasts (WT, n = 3; Rpl5+/−, n = 3).

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