Figure 2.
Rpl5 intronic mutation identified in Skax23m1Jus mice leads to decreased mRNA, protein levels, and polysome defects. (A) Chromatogram of reverse-strand partial sequences of exon 1 and intron 1 of Rpl5 showing the heterozygous mutation (triangle) at the sixth nucleotide in intron 1. The exon-intron junction is indicated by the dotted line. (B) RT-qPCR showed a ∼50% reduction in Rpl5 mRNA in the Rpl5+/− mice when compared with WT ** P< .01. (C) Western blot analysis with vinculin and RPS19 used as loading controls showed reduced levels of RPL5 protein in Rpl5+/− mice when compared with WT. (D) Results from 3 separate experiments indicate Rpl5+/− mice have reduced 60S subunits, 80S ribosomes, and polysomes, consistent with Rpl5 haploinsufficiency.

Rpl5 intronic mutation identified in Skax23m1Jus mice leads to decreased mRNA, protein levels, and polysome defects. (A) Chromatogram of reverse-strand partial sequences of exon 1 and intron 1 of Rpl5 showing the heterozygous mutation (triangle) at the sixth nucleotide in intron 1. The exon-intron junction is indicated by the dotted line. (B) RT-qPCR showed a ∼50% reduction in Rpl5 mRNA in the Rpl5+/− mice when compared with WT ** P< .01. (C) Western blot analysis with vinculin and RPS19 used as loading controls showed reduced levels of RPL5 protein in Rpl5+/− mice when compared with WT. (D) Results from 3 separate experiments indicate Rpl5+/− mice have reduced 60S subunits, 80S ribosomes, and polysomes, consistent with Rpl5 haploinsufficiency.

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