Hemolytic stress augments the hematopoietic phenotype in Rps14^+/− mutants and is rescued by imiquimod. (A) Hemolytic stress experiment. (B-C) Representative views (ventral) of rps14^+/+ and rps14^+/− siblings treated as in panel A and stained with o-dianisidine at 6 dpf. Rps14^+/− mutants demonstrate a clear anemic phenotype, quantified in panel D. (E) Effect of hemolytic stress on HSPCs. Statistical comparisons were performed with Fisher’s exact test (D) or ANOVA (E).