MB1-47 promotes increased glycolysis and glucose flux into the TCA cycle. (A) Schematic diagram of TCA cycle intermediates ¹³C-labeling patterns in cells cultured with [U-¹³C]glucose. (B) Schematic diagram of TCA cycle intermediates ¹³C-labeling patterns in cells cultured with [U-¹³C]glutamine. (C) Isotopologs for the indicated metabolites after 4 hours of [U-¹³C]glucose labeling in DND41 triplicates treated with MB1-47 or DMSO (control). (D) Isotopologs for the indicated metabolites after 4 hours of [U-¹³C]glutamine labeling in DND41 triplicates treated with MB1-47 or DMSO (control). (E) Relative flux activity of TCA cycle in untreated (DMSO) or MB1-47–treated DND41 cells. Statistical significance (P) was determined by using multiple Student t tests. ^#P < .1; *P < .05; **P < .01; ***P < .005. ac-CoA, acetyl coenzyme A; CS, citrate synthase; FH, fumarate hydratase; fwd, forward; GDH, glutamate dehydrogenase; GLS, glutaminase; GOT, glutamic oxaloacetic transaminase; IDH, isocitrate dehydrogenase; α-KG, α-ketoglutarate; MDH, malate dehydrogenase; OGDH, oxoglutarate dehydrogenase; PC, pyruvate carboxylase; PDH, pyruvate dehydrogenase; rv, reverse; SDH, succinate dehydrogenase.