Figure 3.
![Prothrombotic activity of Low Fuc High Gal IgG1 immune complexes is inhibited by Syk, Btk, or P2Y12 inhibition. (A) Volume of thrombi formed in perfusion chambers on VWF plus immune complexes containing spike protein plus either WT IgG or IgG modified to have low fuc high gal after treatment with vehicle (dimethyl sulfoxide [DMSO]), 1 μM R406, 1 μM ibrutinib (Ibr), or 1 μM cangrelor (Cang). (B) Representative images (acquired at 20× magnification) of thrombi stained with DiOC6. Values are mean ± SEM. Significant differences were tested by 2-way ANOVA with the Tukey multiple comparisons test. *P < .05; **P < .01.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/138/16/10.1182_blood.2021011871/2/bloodbld2021011871f3.png?Expires=1724847560&Signature=f9ju1GEhMHRYH5Bo2tFPaoJ8klKC3QjKzpkFv-JzV7uq2viWRvHPCBLt8Z~rRGvVOsZez~j1iDCWMhL2k2sCxYuzjCNuO62~g6uur-kuZh4CbPBQ3WFCfL2YXgmARFJ09V28eVoxxzkbFPDcC7EGTxtuivqniTfCF9THw4fr6EAQlnOk~AAyD8znBvJbgJwUVE5xNkgl~hm4uYr8KuZs3CYJ-VVHbkczID-6wGuLXkghNwI72DJed6TS9qOI9gX2zdRx2zcMwD~7Yy4F~8cECu55GDpIkHJ5QmOv1lgsKD0~BghIGvjsoORAjBbyJiads4f3kdQHHJjefw8ndFI-~g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Prothrombotic activity of Low Fuc High Gal IgG1 immune complexes is inhibited by Syk, Btk, or P2Y12 inhibition. (A) Volume of thrombi formed in perfusion chambers on VWF plus immune complexes containing spike protein plus either WT IgG or IgG modified to have low fuc high gal after treatment with vehicle (dimethyl sulfoxide [DMSO]), 1 μM R406, 1 μM ibrutinib (Ibr), or 1 μM cangrelor (Cang). (B) Representative images (acquired at 20× magnification) of thrombi stained with DiOC6. Values are mean ± SEM. Significant differences were tested by 2-way ANOVA with the Tukey multiple comparisons test. *P < .05; **P < .01.