IK6 knock-in leads to shortened survival time in NSG mice with increased extramedullary organ engraftment. NSG mice xenografts transplanted with Nalm-6 clonal cell lines. (A) Flow cytometry for human HLA-ABC marker in peripheral blood samples taken on day 14. (B) Kaplan-Meier survival curve; n = total number of mice in each group. Statistical significance was calculated by using one-way analysis of variance. (C) Mouse liver weights at time of euthanasia. Statistical significance was calculated by using the Student t test. Drug treatment of Nalm-6 clonal cell lines showing percent annexin V/7-AAD double-negative population as measured by flow cytometry after 72 hours of treatment with dexamethasone (D), cytarabine (E), daunorubicin (F), and asparaginase (G). (H) TaqMan qRT-PCR analysis of relative expression of NR3C1 at baseline and fold change in expression of FKBP5 and TSC22D3 after dexamethasone treatment in Nalm-6 clonal cell lines of indicated genotype. For all experiments, samples were performed in technical triplicate. Error bars show standard error of the mean. *P < .05; **P < .01; ***P < .001. PBS, phosphate-buffered saline.