Follow-up NGS analysis in patients with CIN with clonal disease. The graphs show the mutational status and size of clones in 9 patients with CIN with clonal disease in whom follow-up NGS studies were available (shown in supplemental Table 1). The detected mutations and the respective VAFs (%) at the first evaluation (M0) and subsequent analysis in different time points shown in months (M) are depicted. In 6 patients, the clonal size (VAF) of the acquired mutations remained relatively stable between the first and subsequent NGS analyses (A-E, I). Of the 3 patients with available follow-up NGS who progressed to myeloid neoplasm, the clonal size was increased and additional mutations were detected at the time of progression in 2 of them (panels F and G); in the third patient, only a modest increase was detected in the VAF of mutations, with no additional mutations acquired at the time of progression (H). Panels A-I correspond to patients #7, #15, #1, #20, #8, #16, #21, #2, and #12, respectively, as presented in supplemental Table 1.