GSnP-6 and P-G6 inhibit P-selectin binding to leukocytes. GSnP-6 and P-G6 (0-100 μM) were incubated with mouse (A-B) or human (C-D) neutrophils and monocytes. Flow cytometry was used to evaluate the percentage of binding inhibition of species-appropriate P-selectin chimera to neutrophils or monocytes produced by GSnP-6 and P-G6 compared with phosphate-buffered saline control. GSnP-6 and P-G6 inhibit P-selectin leukocyte interactions in a dose-dependent manner. Data are mean ± SEM, n = 3 per agent per study. PMN, polymorphonuclear cells.