TCR signaling in SASH3-mutated patients. Immunoblots for the indicated proteins and phosphorylated proteins on lysates from 5 × 10³ CD3⁺ T cells (obtained from PBMCs upon negative selection with magnetic beads) with (+) and without (–) stimulation with anti-CD3 and anti-CD28 Dynabeads for 20 minutes from 3 healthy controls and P1 and P2. β-actin was used as loading control.