Patients with T-ALL with high wild-type IL7R expression display evidence of oncogenic IL-7R–dependent signaling activation. (A) Normalized IL7R gene expression levels in T-ALL patients with wild-type IL7R (n = 246). Dashed lines mark the 20 cases with the highest expression (above top line) and the 20 cases with lowest IL7R expression (below bottom line), used for comparison in the subsequent analyses. Only IL7R wild-type cases were analyzed. (B-C) Ranked GSEA on differentially expressed genes between IL7R-high and -low cases for the sets of IL-7 target genes in pro-T cells (B), and genes upregulated in IL7R-mutant T-ALL samples (left) and downregulated in IL7R-mutant cases (right) (C).