Localization of HRG with platelets and fibrin in pulmonary thrombi. Frozen lung sections harvested from wild-type (WT; panels A-C) or HRG^−/− (panels D-E) mice 60 minutes after IP polyP administration were subjected to immunofluorescence using antibodies against HRG, fibrin, and platelets. 4′,6-Diamidino-2-phenylindole (DAPI) was used as a nuclear stain. The combinations for immunostaining were paired as follows: panels A and D, HRG (green) and fibrin/fibrinogen (red); panels B and E, platelets (green) and HRG (red); and panel C, platelets (green) and fibrin/fibrinogen (red). Sections were visualized by using a ×100 objective lens and an Olympus BX41 microscope equipped with a DP72 camera (Olympus, Tokyo, Japan). Scale bars represent 100 μm.