Influence of SF mutations on survival of RUNX1- and ASXL1-mutated patients. (A) Kaplan-Meier curves for EFS in relation to SFmut4 in combination with the mutation status of RUNX1. (B) Kaplan-Meier curves for EFS in relation to the mutation status of SRSF2 in combination with RUNX1. Multivariable Cox regression analysis of EFS in relation to the mutation status of SRSF2 (C) or SFmut4 (D) with RUNX1 mutations and modified ELN 2017 classification. In the modified ELN 2017 classification, RUNX1 and ASXL1 mutations were excluded; patients carrying a RUNX1 or ASXL1 mutation were reclassified based on the presence of the rest of the aberrations in this classification system. Type of stem cell transplantation violated the proportional hazard assumption; therefore, it was used as strata variable in all of the multivariable Cox regression models (for variables used in the model as strata, the statistics were not calculated and do not appear in the results). Because the number of cases for which data on all 4 of the most common SF mutations (included in the SFmut4 variable) were available was smaller than the number of cases for which SRSF2 alone was available, the patient numbers used in the analyses differ in panel B and panel C differ. *P < .05; **P < .01; ***P < .001. HR, hazard ratio; Ref, reference; sAML, sAML/tAML/MDS; 95% CI, 95% confidence interval.