iNKT sublineages display different in vitro suppressive potential. (A) Genome tracks showing a comparison of ATAC-seq and RNA-seq profiles for genes encoding cytokines in iNKT1 (blue), iNKT2 (red), and iNKT17 (green) cells. Data are merged from 2 biological replicates. Black boxes highlight differentially accessible ATAC-seq peaks. In vitro suppression assay of indicated iNKT sublineages. (B-C) Representative profiles (B) and summary (C) of violet tracer dilution as well as CD25 and ICOS expression of CD4 T cells stimulated with anti-CD3/anti-CD28 activation beads in the presence or absence of FACS-sorted iNKT1, iNKT2, and iNKT17 cells. Data are representative of 2 independent experiments. MFI, mean fluorescence intensity.