Analysis of the chromatin accessibility landscape in iNKT sublineages. (A) Volcano plots showing significance and log₂ fold change of ATAC-seq peaks in pairwise comparisons between iNKT sublineages. Peaks colored in gray on volcano plots indicate a log₂ fold change of −2 to +2 and/or an adjusted P value >.05. Peaks with a log₂ fold change of less than −2 or >2 and an adjusted P value <.05 are colored according to the iNKT sublineage represented (iNKT1, blue; iNKT2, red; and iNKT17, green). Vertical dotted lines on volcano plots indicate a log₂ fold change of 2; horizontal dotted line indicates an adjusted P value of .05. (B) Heatmap showing clusters for the top 2500 varying ATAC-seq peaks. Colors indicate z score of reads in each peak scaled to the mean across all iNKT cell subsets and replicates.