AnFIXphylogeny and sequences. (A) Phylogeny and ancestral FIX sequences were constructed using extant FIX sequence information. A phylogram of the tree is shown for display purposes. Closed circles and bold numbers denote node designations for reconstructed AnFIX molecules. (B) Amino acid sequence alignment of AnFIX sequences was generated using Clone Manager 9 software. Sequence variations from hFIX are shown in the lower rows.