Breakpoint characterization in BCL11B-a AL. (A) Break-apart FISH assay differentiating t(2;14), t(6;14), t(7;14), and t(8;14) translocations (fosmid WI2-2168J13, red and fosmid WI2-2934J16, green) from 4 known rearrangements in T-ALL. The thin black horizontal lines represent fosmids that were used to narrow the 14q32 breakpoints. Arrows indicate the mapping of the breakpoint in each case. (B-E) Mapping of breakpoints at CDK6/7q21.2 (B); ZEB2/2q22.3 (C); 6q25.3 (D); 8q24 (E). Patient case numbers refer to Table 1. The figure panels are not to scale. SE, superenhancer.