Comparison of the transcriptomic profiles of WT and mutant-BTK–overexpressing cells. (A-C) Volcano plots comparing MEC-1 cells overexpressing BTK^WT and BTK^C481S (A), BTK^WT and BTK^C481R (B), and BTK^C481S and BTK^C481R (C). The DEGs (FDR, ≤0.05; fold change, >2 or < −2) are labeled in red. (D-F) Heat maps of the DEGs (FDR, ≤0.05; fold change, >2 or <−2) between MEC-1 cells overexpressing BTK^WT and BTK^C481S (D), BTK^WT and BTK^C481R (E), or BTK^C481S and BTK^C481R (F).