MCL cell interaction with macrophages induces M2 phenotype in coculture system. The surface expression of CD163 (A) and CD80 (B) in CD14-Mφ was measured by flow cytometry after treatment with cytokines (IL-4 or lipopolysaccharide (LPS) plus IFN-γ) or CM collected from Mino or Jeko cells or coculturing with Mino or Jeko. The surface expression of CD163 (C) or CD80 (D) in THP-1-Mφ was measured by flow cytometry after coculturing with Mino cells. (E) CD163 expression was measured in the Mino cell lines after coculturing with THP-1-Mφ with or without IL-10 or IL-4 neutralizing antibodies. Experiments were repeated 3 times, and a representative experiment is shown. *P < .05. Ab, antibody.