HK deficiency does not inhibit coagulation activation, but does ameliorate APAP-induced hepatotoxicity. (A) Plasma concentration of TAT complexes at 6 and 24 hours post-APAP administration. (B) Plasma ALT activity. (C) Histologic scoring of liver pathology according to supplemental Table 1. (D) Representative photomicrographs of liver histology from HK^+/+ and HK^−/− animals at 24 hours after APAP treatment, ×40 and ×100 original magnification. A representative pericentral necrotic lesion is outlined in black. N = 26 to 30 mice per group, combined from 6 independent experiments. Saline samples collected at 24-hour time point. N = 9 to 10 mice per group. Error bars represent standard error of the mean. *P < .05, **P < .01, ***P < .001.