Conditional loss of Chd8 in hematopoietic cells leads to hematopoiesis and BM failure. (A) Schematic model of CHD8 deletion in hematopoietic cells. Chd8^Flox mice were crossed with Mx1-Cre mice, and deletion was induced by 4 pI/pC injections. (B) Western blot confirmation of CHD8 protein loss after 6 days of pI/pC injections in BM progenitors. The quantification represents data from 3 repeat experiments. (C) PB counts of Chd8^Flox and Chd8^Flox;Mx1-Cre mice after 6 days of pI/pC induction by Hemavet. ***P < .001. (D) Representative H&E staining of femurs from WT and Chd8^−/− mice which were harvested at 6 days after deletion. WT indicates Chd8^F/F mice. (E) Total BM cells count with Hemavet after 6 days of pI/pC injections. (F) Number of mature lineage cells in WT vs Chd8^−/− mice. WT vs Chd8^−/− (E-F). ***P < .001.