BATF overexpression increases sensitivity to venetoclax. (A) Transcription factor motifs enriched in resistant cell lines. (B) Genome-wide E2A transcription factor footprint. (C) Transcription factor motifs enriched in sensitive cell lines. (D) Genome-wide BATF transcription factor footprint. (E) Protein expression of BATF, BIM, MCL1, BCLXL, BCL2, and ACTIN in cell lines expressing 2 independent control guides (cnt.1 and cnt.2) or 2 independent guides to BATF (BATF.1 and BATF.2) for CRISPR activation in dCAS9-VP64 expressing KMS11. (F) Cells were treated with indicated doses of venetoclax for 24 hours followed by staining with Annexin V–fluorescein isothiocyanate to measure apoptosis. Data are presented as the mean ± standard error of 3 independent experiments. Statistics were calculated by using two-way analysis of variance followed by Dunnett’s multiple comparison test. *P < .05; ***P < .001; ****P < .0001.