Increased expression of B-cell markers in venetoclax-sensitive myeloma. RNA-sequencing heat map of genes with more than twofold differential expression and FDR <0.05 from cell lines (A) and patient samples (B). Sensitive (green) and resistant (orange) cell lines are denoted by colored boxes above the heat map. Presence of t(11;14) or t(4;14) is indicated by black boxes. To the left of the heat map, genes overexpressed in sensitive cell lines are labeled in green, and genes overexpressed in resistant lines are labeled in orange. (C) Enrichment of upregulated genes from venetoclax-sensitive myeloma cell lines in venetoclax-sensitive patient samples by GSEA. (D) Scatter plot of all genes and corresponding log twofold change in sensitive vs resistant patient samples (x-axis) and cell lines (y-axis). Genes statistically significant in both data sets or one data set are highlighted in red or black, respectively. (E) RNA expression of BCL2, BCL2L1, and MCL1 as well as calculated BCL2/BCL2L1 and BCL2/MCL1 RNA ratios in sensitive (green) and resistant (orange) cell lines. (F) Enrichment of B-cell signature genes and the Zhan CD2 expression signature in venetoclax-sensitive cell lines and patient samples by GSEA. FC, fold change; GX, gene expression; NES, normalized enrichment score.