HSC niche signature is enriched in EV-primed BM-MSCs. (A) Hierarchical clustering of untreated, TNF/LT-primed, and EV-primed BM-MSCs on selected differentially expressed genes as evaluated by RNA-seq. The relative level of gene expression is depicted according to the shown color scale. (B) qPCR validation of the RNA-seq data for BM-MSCs treated by FL-derived EVs (n = 27) and TNF/LT (n = 9) highlighting genes upregulated by EVs (left) or TNF/LT (right). Results are expressed as the fold change compared with untreated BM-MSCs assigned at 1 (red dotted line). *P < .05; ***P < .001.