Functional CMV IE-1 CD8⁺T-cell subsets at the patient level grouped by late CMV reactivation. Heat map of functional CD8⁺ T-cell subsets to CMV IE-1 at the subject level quantified by posterior probabilities and grouped according to late CMV reactivation (lower violet) vs no reactivation (upper green). Rows correspond to entire antigen-specific cell subsets represented in a patient and are arranged in columns by ascending degree of functionality, from 1 (light blue) to 5 (pink) functions. Individual heat map cells show posterior probability that a functional antigen-specific cell subset is present in a patient and are color coded from white (0) to dark purple (1). For example, patient 1 has a probability of 1.0 of possessing CD8⁺ T cells that express all 5 functional markers but 0 probability of possessing CD8⁺ T cells expressing only TNF-α and granzyme B in response to stimulation with IE-1. Note the increased proportion of subjects with 4 and 5 functional type CD8⁺ T-cell subsets (red box) among patients without CMV reactivation vs those with CMV reactivation.