LRP-1 mediates the effects of tPA on neutrophil recruitment and NET formation after ischemic stroke. (A-B) Representative immunoblots and quantitative determinations of LRP-1 expression in the ischemic cortex at 24 hours after stroke in mice treated with vehicle or tPA compared with mice undergoing sham surgery (n = 5). (C) Representative confocal images of LRP-1 immunostaining in the ischemic cortex. DNA was stained with 4′,6-diamidino-2-phenylindole (blue). Independent experiments are repeated ≥3 times. Scale bar, 40 μm. (D) Quantification of MPO activity in the ischemic brain (n = 6). (E) Representative immunoblots of H3Cit in the ischemic cortex. (F) Quantification of MPO activity in the ischemic brain (n = 6). (G) Representative immunoblots of H3Cit in the ischemic cortex. (H) Representative confocal images of fibrin intravascular deposits (green) and CD31⁺ microvessels (red) in the infarct areas at 24 hours. Scale bar, 10 μm. (I) Quantification of fibrin intravascular deposits for each group (n = 6). Values are means ± standard deviation. *P < .05. NS, not significant; TXA, tranexamic acid.