Gata2b deficiency results in decreased myeloid differentiation in adult zebrafish KM. (A) Gating strategy of fluorescence-activated cell sorting analysis of whole KM of WT and gata2b^−/− zebrafish. Percentages represent the average of all zebrafish analyzed per genotype. (B-C) Quantitation as percentages of single viable cells over time of myeloid (B) and lymphoid (C) and HSPC populations. (D) Representative example of Tg(mpx:GFP) expression in WT and gata2b^−/− zebrafish KM by fluorescence microscopy. (E) Forward (FSC) and side scatter (SSC) profiles of Tg(mpx:GFP) expression in WT and gata2b^−/− zebrafish KM in green. (F) Quantitation of Tg(mpx:GFP)⁺ cells expressed as percentage in single viable cells. Each dot represents KM analysis of 1 zebrafish. (G) Representative figure of sorted Tg(mpx:GFP)⁺ cells from WT and gata2b^−/− zebrafish KM after May Grunwald-Giemsa (MGG) staining. (H) Quantification of Tg(mpx:GFP)⁺ cells from WT and gata2b^−/− zebrafish KM based on the differentiation phenotype using MGG staining. (I) Gating strategy for CD41:GFP^low-expressing cells in total KM in green. (J) FSC and SSC plots of WT and gata2b^−/− KM cells and CD41:GFP^low-expressing cells in green. (K) Quantification of the frequency of CD41:GFP^low cells in single live cells of total KM. Each dot represents KM analysis of 1 zebrafish. (L) FSC/GFP scatter profile of Tg(IgM:GFP) WT KM. (M) Quantitation of gating 1 of Tg(IgM:GFP) WT and gata2b^−/− KM as percentage of single viable cells. Each dot represents KM analysis of 1 zebrafish. (N) Quantitation of gating 3 of Tg(IgM:GFP) WT and gata2b^−/− KM as percentage of single viable cells. Each dot represents KM analysis of 1 zebrafish. (O-R) Representative image of sorted IgM:GFP⁺ cells indicating lymphoplastic cell (O), lymphocyte (P), phagocytic B cell (Q), and plasma cell (R). (S) Quantitation of sorted IgM:GFP⁺ cells per genotype. Error bars represent standard error of the mean. *P < .05, **P < .01, ***P < .001, ****P < .0001.