Single-cell epigenetic and transcriptional analyses reveal epigenetic changes regulating gene expression with gata2b loss. Scatter plots of gene expression change in scRNA-seq (log₂ fold-change, gata2b^ko vs WT) and genescore change in scATAC-seq (log₂ fold-change, gata2b^ko vs WT) in (A) HSPC-MPPs (HSPC/thromb vs HSPC-MPP), (B) myeloid progenitors (MyelProg1 vs Myeloid progenitor), and (C) lymphoid progenitors (LymphProg vs HPC). P values are shown for linear regression analysis. (D) Gene annotation and pathway identification within overlap genes between GATA2 bound genes in CD34⁺ cells via ChIP-sequencing (11 151 genes) and differentially expressed (DE) genes in lymphoid progenitors (LP) with sc-RNA-seq. Of 298 DE genes in LP, 257 with human orthologs were compared. Within the “hemopoiesis” gene ontology (GO) term, the gene list included those critical in B lymphopoiesis.