gata2b^kozebrafish have increased lymphoid priming with incomplete B-cell development. (A) Quantification of mature hematopoietic cell types from scRNA-seq in WT and gata2b^ko zebrafish within the erythrocyte-deplete marrow. *P < .05, Fisher’s exact test. (B) Volcano plots of differential expression of genes within lymphoid progenitor cells between WT and gata2b^ko zebrafish. Dashed lines depict log₂ fold change of ±0.6 and −log₁₀ (P value) of 1.3. (C) Enrichment plot of oxidation-phosphorylation signature found highly depleted among differentially regulated genes of gata2b^ko lymphoid progenitor cells by GSEA. NES, normalized enrichment score.