scRNA-seq reveals loss of monocytic program in gata2b^kozebrafish. (A) Split UMAP of marrow cells in WT (n = 3) and gata2b^ko (n = 3) sorted marrow cells (inDrops). ErythProg, erythroid progenitor; LymphProg, lymphoid progenitor; MyelProg1 and 2, myeloid progenitor; Thromb, thrombocytes. (B) Quantification of immature hematopoietic cell types from scRNA-seq in WT and gata2b^ko zebrafish within the erythrocyte-deplete marrow. *P < .05, Fisher’s exact test. (C) Volcano plots of differential expression of genes within HSPC and myeloid progenitor 2 populations between WT and gata2b^ko zebrafish. Dashed lines depict log₂ fold change of ±0.6 and −log₁₀ (P value) of 1.3. NK, natural killer.