Pyroptosis of monocytes and macrophages is responsible for DVT. (A-D) WT (n = 21) and GSDMD-deficient mice (n = 20) were subjected to IVC stenosis. Thrombus prevalence (A), thrombus weight (B), and thrombus length (C) were measured at 48 hours after the surgery. Representative images of thrombi are shown in panel D. Lines represent the medians. ^⋆P < .05; ^⋆⋆P < .01. The χ² method was used in panel A, and Mann-Whitney U test was used in panels B and C. (E) Western blot analysis of clots from WT, caspase-1, and GSDMD-deficient mice. Fibrin in the same amount of tissue lysates was detected by immunoblot with an antifibrin monoclonal antibody (59D8). (F) Fibrin in the same amount of proteins of the tissue lysates from WT and caspase-11–deficient mice was detected by immunoblot with an antifibrin monoclonal antibody (59D8). (G-I) Effect of monocyte depletion on venous thrombosis. C57BL/6J mice administered with GdCl₃ (n = 13) or buffer (n = 17) were subjected to IVC stenosis. Thrombus prevalence (G), thrombus weight (H), and thrombus length (I) were measured at 48 hours after the surgery. Lines represent the medians. ^⋆P < .05. The χ² method was used in panel G, and Mann-Whitney U test was used in panels H and I. Ctrl, control.