Osteocytes stimulate neutrophil development in vitro and in vivo. (A) FACS analysis of myeloid progenitor cell populations of BM in 12-week-old Dmp1-TSC1 and control mice (mean ± SD; n = 10). (B) FACS analysis of myeloid progenitor cell populations of BM in 12-week-old Dmp1-Rheb and control mice (mean ± SD; n = 10). (C) FACS analysis of LSK cells of BM in 12-week-old Dmp1-TSC1 and control mice (mean ± SD; n = 10). (D) FACS analysis of LSK cells of BM in 12-week-old Dmp1-Rheb and control mice (mean ± SD; n = 10). (E) FACS analysis of CD11b⁺Gr-1⁺ and CD11b^intGr-1^int (PM/MC neutrophil) cell proliferation in the BM of 12-week-old Dmp1-TSC1 and control mice (mean ± SD; n = 10). (F) FACS analysis of CD11b⁺Gr-1⁺ and CD11b^intGr-1^int cell proliferation in the BM of 12-week-old Dmp1-Rheb and control mice (mean ± SD; n = 10). (G) BM cells from 12-week-old C57BL/6 mice were cultured in conditioned medium (CM) from osteoclasts, osteocytes, monocytes, BMSCs, lymphocytes, or endothelial cells for 10 days. FACS analysis of CD11b⁺Gr-1⁺ cell proliferation (mean ± SD; n = 3). Data are mean ± SD of 3 independent experiments. **P < .01; ***P < .001.