Electron microscopy analysis of SCD RBCs. Ultrastructural analysis of RBCs using electron microscopy. RBCs from patient who was SCD-Baseline were processed using FIB-SEM. (A) Electron micrograph of peripheral blood erythrocytes from a patient with SCD showing mitochondria. Scale bars, 2 μM. (B) Three-dimensional rendering of mitochondria inside the sickle erythrocyte. Image segmented mitochondria are red. Scale bars, 10 μM. (C) Series of image frames from the FIB-SEM image stack. Slice thickness, 9 nm. Isotropic voxel size, 9 nm. (D) Outward morphology of sickle erythrocyte. Cell periphery, green. Scale bars, 10 μm.