Enumeration of and expression of anti-apoptotic BCL-2 family proteins by mouse neutrophils recovered after culture. (A) B6 (wild type) or BAX^−/−BAK^−/− viable neutrophil (PMN) recovery was assessed before or after culture in standard media (B) B6 neutrophils were cultured with or without GM-CSF or G-CSF at the indicated dose for 16 hours before the number of viable PMN was determined by flow cytometry. Data are shown as mean ± SD of viable PMN per well. Data show a single representative experiment of n = 3 experiments. (C) Western blot analysis was performed on freshly isolated B6 mouse neutrophils (T0) or those cultured with 5 ng/mL G-CSF (+G) or 5 ng/mL GM-CSF (+GM). Data from n = 3 independent experiments with protein expression displayed relative to actin loading control. Data analyzed using a 2-tailed Student t test. *P < .05, **P < .01, ***P < .001.