Multiple orthogonal single-cell methods reveal site-to-site heterogeneity in FL. (A) Summary plot showing the degree of site-to-site dissimilarity for each single-cell method (symbols) for each patient (FL1-FL10). Site-to-site dissimilarity is quantified by Renkonen SI for scBCR-seq, scRNA-seq, and flow cytometry and by site-to-site abundance ratio for Tfh cells. (B) Model illustrating (I) cases with divergent clonal evolution and little cell migration leading to significant site-to-site heterogeneity, including in recruitment and co-option of Tfh cells, compared to (II) cases with high degree of tumor cell migration between sites, leading to increased sharing of evolving subclones.