Site-to-site heterogeneity in the TME. (A) Fraction of 50 most abundant TCRβ clonotypes shared between tumor sites A and B. Dots are colored by patient identity as in Figure 2A. (B) Fraction of all T cells for each tumor site (tumor site A and site B) per patient (FL1-FL10), belonging to each T-cell subpopulation (left). Bars are colored by T-cell subpopulation as in Figure 5A. Spearman correlation between site-to-site abundance ratio of each T-cell subpopulation and tumor cell dissimilarity (quantified by Renkonen SI) (right). (C) Spearman correlation between site-to-site abundance ratio for Tfh cells and tumor cell dissimilarity (quantified by SI). Dots are colored by patient identity. (D) Spearman correlation of CD40 expression in tumor cells and MKI67 expression in Tfh cells of all 20 tumor samples. Dots are colored by patient identity.