Characterization of the TME. (A) UMAP plot of all T cells from 20 tumors from 10 patients. Clusters are denoted by color and labeled according to phenotype. (B) Heatmap of the top 30 differentially expressed genes (rows) between T-cell subpopulations (columns; n = 3600 cells); the Wilcoxon test with Bonferroni correction was used. Selected canonical markers are highlighted on the right side. Bar above is colored by cluster as in panel A. (C) UMAP plot of non-T cells from 20 tumors from 10 patients. Clusters are denoted by color and labeled according to phenotype. (D) Heatmap of the top 30 differentially expressed genes (rows) between non-T subpopulations (columns; n = 2098 cells); the Wilcoxon test with Bonferroni correction was used. Selected canonical markers are highlighted on the right side. Bar above is colored by cluster as in panel C. FDC, follicular dendritic cell; pDC, plasmacytoid dendritic cell.