Phylogenetic analysis of BCR-defined subclones reveals site-specific patterns. (A) Representative phylogenetic trees based on immunoglobulin heavy chain VDJ sequences from single-cell BCR sequencing of tumor samples from both sites from 2 patients with site-specific BCR subclones (FL2, FL8) and from 2 patients with shared BCR subclones (FL1, FL10). The sampled malignant lymph node sites (site A in orange, site B in purple) are visualized on the left for each patient. The bar below the phylogenetic tree reveals the site of origin for each BCR sequence in the tree. Similarity index (SI) according to Renkonen is given on the top right for each patient. (B) Representative trajectory analysis of tumor cells from scRNA-seq of tumor samples from both sites (site A and site B) from 2 patients with site-specific BCR subclones (FL2, FL8) and from 2 patients with shared BCR subclones (FL1, FL10). The cells are colored by tumor site.