Patterns of clonal evolution in patients with refractory disease. Patterns of clonal evolution in refractory (RD) patients with loss of FLT3-ITD at RD (A-B) or persistent FLT3-ITD at RD (C-D). VAFs are given on the left of each graph and the allelic ratios of FLT3-ITDs on the right. Persistent mutations are color-coded in yellow, lost mutations in blue, and gained or upregulated mutations in green. FLT3-ITDs are indicated by dotted lines.