Pyruvate but not in situ glycolysis is required for mitochondrial metabolism during murine erythroblast enucleation. (A-C) Graphs displaying the viability (top), enucleation rate (middle), and mitochondrial activity (bottom) of orthochromatic erythroblasts in the presence or absence of glucose (10 mM), glutamine (4 mM), pyruvate (4 mM), dimethyl α-ketoglutarate (MOG; 1 mM), or methyl pyruvate (MP; 1 mM) (B) or lactate (2 mM) determined in the ex vivo enucleation assay at the 18-hour time point. (D) Graphs of the viability (top) and enucleation rate (bottom), of orthochromatic erythroblasts in the presence of dimethyl sulfoxide (DMSO) control or lactate dehydrogenase inhibitor (NHI-2) at indicated concentrations determined in the ex vivo enucleation assay at the 18-hour time point. Mean ± standard error of the mean (n ≥ 3). *P < .05, **P < .01, ***P < .001, ****P < .0001 by Student t test.