Figure 7.
![Effect of drugs on megakaryopoiesis in RUNX-1in adult CD34+ HSPCs. Experiments are as described in Figure 6, but with added J-IN8, RS, GS, and DAPT. Mean ±1 SEM (n = 4 experiments per study arm; P values by 2-way analysis of variance [ANOVA]). (A) Drug effects correcting the Mk yield per HSPC of NT vs RUNX1 shRNA lentiviral-transduced CD34+ HSPCs after exposure to J-IN8 (0.1 μM), RS (0.1 μM), GS (0.1 μM), or DAPT (10 μM), added on days 5 to 14. (B-D) Effect of RS on colony-forming assays performed using sorted NT- and RUNX1 shRNA-expressing CD41− and CD41+ HPSCs. Sorted cells were seeded in MegaCult medium for Mk colonies (B) or MethoCult medium for erythroid (C) and GM colonies (D).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/137/19/10.1182_blood.2020006389/2/bloodbld2020006389f7.png?Expires=1730485300&Signature=3qwm1NoAZYGpWLYTwnd5POXqRWKWlbq4z4EpcYgFdVLxRyLkawBd2M73Bb-tsLw4OO1xfHVt9h6trfumMW~pzivQHmIF07OlS~JC~jfnKGSIYt~0Q1fcFLmDpI5I31h~qoISYLKSB9eQOAwPA6a8leZbpIEn38RlA4GqUvxWkyFB6vT4INZ2CWj7-ZnTis65-oZqnSVJIBQvBbyT6SFOtijJ1AbiPUZ5v9fd827XvpjCTTlUFHNPX7Z8MGuNBEmxGkXepHoWaxLA9eoQ7GgRqmFNLpDbMwr5hip1CvnYUMNQGPEzrwpDOm1ebTpUQsuOMnxIXdY8Lm-TF~FH1CnWyA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of drugs on megakaryopoiesis in RUNX-1in adult CD34+ HSPCs. Experiments are as described in Figure 6, but with added J-IN8, RS, GS, and DAPT. Mean ±1 SEM (n = 4 experiments per study arm; P values by 2-way analysis of variance [ANOVA]). (A) Drug effects correcting the Mk yield per HSPC of NT vs RUNX1 shRNA lentiviral-transduced CD34+ HSPCs after exposure to J-IN8 (0.1 μM), RS (0.1 μM), GS (0.1 μM), or DAPT (10 μM), added on days 5 to 14. (B-D) Effect of RS on colony-forming assays performed using sorted NT- and RUNX1 shRNA-expressing CD41− and CD41+ HPSCs. Sorted cells were seeded in MegaCult medium for Mk colonies (B) or MethoCult medium for erythroid (C) and GM colonies (D).