Role of thrombin-driven fibrin polymer formation in hepatic fibrin(ogen) cross-linking after APAP overdose. Mice expressing mutant fibrinogen^AEK (Fib^AEK mice) and WT mice were treated intraperitoneally with saline vehicle or 300 mg/kg APAP. Livers were collected 24 hours after APAP challenge. (A) Fibrin(ogen) labeling in frozen liver sections was quantified (n = 11-12 mice/group). (B) Representative photomicrographs of fibrinogen-labeled frozen sections (×5 virtual magnification). Fibrin(ogen) levels were measured in enriched insoluble liver extracts using capillary-based western blotting (Wes). (C-E) Representative digital capillary images show fibrin(ogen) detected and quantified (F-H) by rabbit polyclonal antibodies selective for fibrin(ogen) Aα, Bβ, and γ chains. n = 4 mice for vehicle-treated groups and 11 to 12 mice for each APAP-challenged group (F-H). Data expressed as mean ± standard error of the mean. *Significantly (P < .05) different from vehicle-treated mice of the same genotype. ^#Significantly (P < .05) different from APAP-challenged WT mice.