Deposition of cross-linked fibrin(ogen) in livers of mice after APAP overdose. WT mice were treated intraperitoneally with saline vehicle (Veh) or 300 mg/kg APAP, and hepatic fibrin(ogen) levels were determined 24 hours after challenge. (A-B) Representative photomicrographs (200×) show H&E-stained (A) and fibrin(ogen)-labeled (B, brown) liver sections. Necrosis denoted by black arrowhead. (C-I) Fibrin(ogen) levels were measured in enriched insoluble liver extracts using capillary-based western blotting (Wes). (C-F) Digital capillary images show fibrin(ogen) detected by rabbit polyclonal antibodies against fibrinogen (C) and selective for fibrin(ogen) Bβ (D), γ (E), and Aα chains (F). (G-I) Quantification of peaks indicated by the # in panels D-F is shown (n = 3 mice/group). Data are expressed as mean ± standard error of the mean. *Significantly (P < .05) different from saline-treated mice.