Effect of COMMD7 KO on platelet functional responses in MKs. (A-B,D-F) Negative control or COMMD7 KO day 13 MKs were kept at baseline or stimulated with convulxin (2 μg/mL) or thrombin (1 U/mL) for 3 or 10 minutes as indicated and analyzed by flow cytometry. Viable MKs were gated as in Figure 2B. Shown is the normalized mean ± SEM of percent positive cells for PAC-1 (A), labeled fibrinogen (B), surface P-selectin (D-E) , or surface CD63 (F) (3-5 independent cords per group). (C) Mean ± SEM of the percentage of control or CRISPR KO MKs spread after 1 hour on collagen or fibrinogen. Shown are samples from 3 cords with each sample plated in duplicate wells; >20 fields and >200 MKs were scored (blinded to treatment) per sample. Paired Student t tests: *P < .05; **P < .01.