C/EBPα interferes with GATA1-dependent metabolic program. (A) Expression of C/EBPα protein in TF1a and HEL. It is noteworthy that C/EBPα was detectable in TF1a but not in HEL cells under basal conditions (left). Intensities of C/EBPα bands were quantified by densitometry and normalized to those for β-tubulin (right). (B) Increased GATA1 in CEBPA-KD TF1a cells. CEBPA-KD upregulated GATA1 protein. (C) Expression changes of LSD1 target genes in CEBPA-KD TF1a cells. (D) Increased enrichment of GATA1 at the ALAS2 and GLUT1 enhancers in CEBPA-KD TF1a cells. (E) Increased glucose uptake in CEBPA-KD TF1a cells. (F) Expression changes of LSD1 target genes in S2101 treated and CEBPA-KD TF1a cells. Concentration of S2101 is 50 µM. (G) GATA1 enrichments at ALAS2 and GLUT1 enhancers in S2101-treated and CEBPA-KD TF1a cells. Enrichment values at the enhancers were normalized to input DNA for panels D and G. (H) Restored glucose uptake by CEBPA-KD in S2101-treated TF1a cells. S2101 was used at 20 µM. Values are mean ± standard deviation of triplicate results. *P < .05, **P < .01.