Hematopoietic potential of HEP from patient-specific iPSC may be reduced. (A) Experimental scheme. HEP (VE-Cad⁺CD73⁻CD43⁻CD235a⁻ cells) were isolated by FACS on day 9 and cultured for additional 5 days on OP-9 mouse bone marrow stromal cells with addition of cytokines for 5 days, at which time hematopoietic potential was assessed by measuring CD43⁺ and CD45⁺ cells by flow cytometry. (B) Hematopoietic potential (%CD43⁺ and/or CD45⁺ cells) of HEP after 5 days of OP-9 coculture is shown. Minimum of 2 independent experiments were performed except for 3 Int5F clones that were differentiated in 1 experiment (control, n = 8; ZF, n = 6; Int5F, n = 3; P = nonsignificant (NS), unpaired Student t test was performed between control and ZF or control and Int5F). NS P values are not shown.