Figure 7
Effect of FXIIa on HRG binding to heparin. Biotin-heparin was adsorbed onto a streptavidin-coated sensor chip to approximately 250 RU. HRG (250nM) was injected in the presence of 10μM ZnCl2, and the association to the chip surface was monitored for 110 seconds before injection of buffer to monitor dissociation. After regenerating the chip, subsequent injections contained 250nM HRG in the presence of 10, 25, 50, or 100nM FXIIa. The binding RU were corrected for background and plotted versus the time course for individual injections. The FXIIa concentrations are indicated above each tracing.